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    Ku80 Recombinant Rabbit mAb (bsm-52512R)  
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    50ul/1400.00元
    100ul/2500.00元
    大包裝/詢(xún)價(jià)

    產(chǎn)品編號 bsm-52512R
    英文名稱(chēng) Ku80 Recombinant Rabbit mAb
    中文名稱(chēng) DNA修復酶Ku-80重組兔單抗
    別    名 86 kDa subunit of Ku antigen; ATP dependent DNA helicase 2 subunit 2; ATP dependent DNA helicase II 80 kDa subunit; ATP dependent DNA helicase II 86 Kd subunit; ATP dependent DNA helicase II; ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80 kDa subunit; CTC box binding factor 85 kDa; CTC box-binding factor 85 kDa subunit; CTC85; CTCBF; DNA repair protein XRCC5; KARP 1; KARP1; Ku 80; Ku autoantigen 80kDa; Ku86; Ku86 autoantigen related protein 1; KUB 2; KUB2; Lupus Ku autoantigen protein p86; NFIV; Nuclear factor IV; Thyroid lupus autoantigen; Thyroid-lupus autoantigen; TLAA; X ray repair complementing defective repair in Chinese hamster cells 5(double strand break rejoining); X-ray repair complementing defective repair in Chinese hamster cells 5(double-strand-break rejoining); X-ray repair cross-complementing protein 5; Xray repair complementing defective repair in Chinese hamster cells 5; XRCC 5; XRCC5; XRCC5_HUMAN.  
    研究領(lǐng)域 染色質(zhì)和核信號  
    抗體來(lái)源 Rabbit
    克隆類(lèi)型 Recombinant
    克 隆 號 1F1
    交叉反應 Human
    產(chǎn)品應用 WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,ICC/IF=1:50-200
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    理論分子量 83 kDa
    檢測分子量
    細胞定位 細胞核 
    性    狀 Liquid
    濃    度 1mg/ml
    免 疫 原 A synthesized peptide derived from human Ku80: 700-732/732 
    亞    型 IgG
    純化方法 affinity purified by Protein A
    緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    PubMed PubMed
    產(chǎn)品介紹 The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008]

    Function:
    Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.

    Subunit:
    Heterodimer of a 70 kDa and a 80 kDa subunit.

    Subcellular Location:
    Nucleus. Chromosome.

    Similarity:
    Belongs to the ku80 family.
    Contains 1 Ku domain.

    SWISS:
    P13010

    Gene ID:
    7520

    Database links:

    Entrez Gene: 7520 Human

    Entrez Gene: 22596 Mouse

    Omim: 194364 Human

    SwissProt: P13010 Human

    SwissProt: P27641 Mouse

    Unigene: 388739 Human

    Unigene: 246952 Mouse



    Ku80也是一種DNA修復蛋白,當細胞在受到輻射損傷而發(fā)生DNA雙鏈斷裂時(shí),Ku80可迅速將其修復,從而提高細胞存活率。
    Ku是一種多功能的蛋白,在許多重要的細胞生命過(guò)程中起著(zhù)直接或間接的作用,如DNA雙鏈斷裂的修復,免疫球蛋白和T細胞受體V(D)J重排,免疫球蛋白構型轉換,DNA復制,DNA轉錄的調節,同時(shí)在細胞周期的G2和M時(shí)相中起著(zhù)特殊的作用。
    產(chǎn)品圖片
    25 ug total protein per lane of various lysates (see on figure) probed with Ku80 monoclonal antibody, unconjugated (bsm-52512R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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