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    HSC70 Mouse mAb (bsm-33211M)  
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    50ul/1180.00元
    100ul/1980.00元
    200ul/2800.00元
    200ug(PBS only)/5600.00元
    大包裝/詢(xún)價(jià)

    產(chǎn)品編號 bsm-33211M
    英文名稱(chēng) HSC70 Mouse mAb
    中文名稱(chēng) 熱休克蛋白71單克隆抗體
    別    名 2410008N15Rik; Constitutive heat shock protein 70; Heat shock 70 kDa protein 8; Heat shock 70kD protein 10; Heat shock 70kD protein 8; Heat shock 70kDa protein 8; Heat shock cognate 71 kDa protein; Heat shock cognate protein 54; Heat shock cognate protein 71 kDa; Heat shock protein 8; Heat shock protein A8; Heat-shock70-KD protein 10, formerly; HSC54; HSC71; Hsc73; HSP71; HSP73; HSP7C_HUMAN; HSPA10; HSPA8; LAP1; Lipopolysaccharide associated protein 1; LPS associated protein 1; LPS associated protein; MGC102007; MGC106514; MGC114311; MGC118485; MGC131511; MGC29929; N-myristoyltransferase inhibitor protein 71; NIP71; HSC 70; HSC-70.  
    研究領(lǐng)域 腫瘤  細胞生物  免疫學(xué)  信號轉導  轉錄調節因子  
    抗體來(lái)源 Mouse
    克隆類(lèi)型 Monoclonal
    克 隆 號 8C7
    交叉反應 Human,Mouse,Rat
    產(chǎn)品應用 WB=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    理論分子量 71 kDa
    檢測分子量
    細胞定位 細胞核 細胞漿 細胞膜 
    性    狀 Liquid
    濃    度 1mg/ml
    免 疫 原 Recombinant human HSC70 Protein 
    亞    型 IgG
    純化方法 affinity purified by Protein G
    緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    PubMed PubMed
    產(chǎn)品介紹 This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

    Function:
    Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones.

    Subunit:
    Interacts with HSPH1/HSP105. Interacts with IRAK1BP1 (By similarity). Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with PACRG and TSC2. Interacts with BAG1. Interacts with SV40 VP1. Interacts with DNAJC7 (By similarity). Interacts with HERC5. Interacts with CITED1 (via N-terminus); the interaction suppresses the association of CITED1 to p300/CBP and Smad-mediated transcription transactivation.

    Subcellular Location:
    Cytoplasm. Melanosome.

    Tissue Specificity:
    Ubiquitous.

    Post-translational modifications:
    Phosphorylated upon DNA damage, probably by ATM or ATR. ISGylated.

    Similarity:
    Belongs to the heat shock protein 70 family.

    SWISS:
    P11142

    Gene ID:
    3312

    Database links:

    Entrez Gene: 3312 Human

    Entrez Gene: 15481 Mouse

    Entrez Gene: 24468 Rat

    Omim: 600816 Human

    SwissProt: P11142 Human

    SwissProt: P63017 Mouse

    SwissProt: P63018 Rat

    Unigene: 180414 Human

    Unigene: 290774 Mouse

    Unigene: 336743 Mouse

    Unigene: 351377 Mouse

    Unigene: 412745 Mouse

    Unigene: 120392 Rat

    Unigene: 201298 Rat



    產(chǎn)品圖片
    Sample: Lane 1: Testis (Mouse) Lysate at 40 ug Lane 2: Kidney (Mouse) Lysate at 40 ug Lane 3: Stomach (Mouse) Lysate at 40 ug Lane 4: Pancreas (Mouse) Lysate at 40 ug Lane 5: NIH/3T3 (Mouse) Cell Lysate at 30 ug Lane 6: Testis (Rat) Lysate at 40 ug Lane 7: Stomach (Rat) Lysate at 40 ug Lane 8: Pancreas (Rat) Lysate at 40 ug Lane 9: 293T (Human) Cell Lysate at 30 ug Lane 10: MCF-7 (Human) Cell Lysate at 30 ug Lane 11: Hela (Human) Cell Lysate at 30 ug Lane 12: A549 (Human) Cell Lysate at 30 ug Primary: Anti-HSC70 (bsm-33211M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 70 kD Observed band size: 68 kD
    Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (HSC70) Monoclonal Antibody, Unconjugated (bsm-33211M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
    Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (HSC70) Monoclonal Antibody, Unconjugated (bsm-33211M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
    Paraformaldehyde-fixed, paraffin embedded (Human stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (HSC70) Monoclonal Antibody, Unconjugated (bsm-33211M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
    Paraformaldehyde-fixed, paraffin embedded (Human duodenum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (HSC70) Monoclonal Antibody, Unconjugated (bsm-33211M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
    Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (HSC70) Monoclonal Antibody, Unconjugated (bsm-33211M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
    Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (HSC70) Monoclonal Antibody, Unconjugated (bsm-33211M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
    Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (HSC70) Monoclonal Antibody, Unconjugated (bsm-33211M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
    Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (HSC70) Monoclonal Antibody, Unconjugated (bsm-33211M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
    Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSC70) Monoclonal Antibody, Unconjugated (ascites of bsm-33211M 8C7) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
    Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSC70) Monoclonal Antibody, Unconjugated (ascites of bsm-33211M 8C7) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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