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    CD5 Mouse mAb (bsm-33077M)  
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    產(chǎn)品編號 bsm-33077M
    英文名稱(chēng) CD5 Mouse mAb
    中文名稱(chēng) CD5單克隆抗體
    別    名 T-cell surface glycoprotein CD5; Lymphocyte antigen 1; Ly-1; Lyt-1; CD-5; CD5 antigen; CD 5; CD5 molecule; CD5 antigen(p56 62); CD5_HUMAN; LEU 1; LEU1; Ly12; LyA; Lymphocyte Antigen CD5; Lymphocyte antigen T1/Leu 1; Lymphocyte antigen T1/Leu-1; Lymphocyte glycoprotein T1/Leu1; OTTHUMP00000236973; p56 62; T1.  
    研究領(lǐng)域 免疫學(xué)  細胞表面分子  t-淋巴細胞  
    抗體來(lái)源 Mouse
    克隆類(lèi)型 Monoclonal
    克 隆 號 12A8
    交叉反應 Human
    產(chǎn)品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/Test
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    理論分子量 55 kDa
    檢測分子量
    細胞定位 細胞膜 
    性    狀 Liquid
    濃    度 1mg/ml
    免 疫 原 KLH conjugated synthetic peptide derived from human CD5 
    亞    型 IgG
    純化方法 affinity purified by Protein G
    緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    PubMed PubMed
    產(chǎn)品介紹 This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]

    Function:
    May act as a receptor in regulating T-cell proliferation.

    Subunit:
    Interacts with CD72/LYB-2. Interacts with PTPN6/SHP-1.

    Subcellular Location:
    Cell membrane; Single-pass type I membrane protein.

    Post-translational modifications:
    Phosphorylated on tyrosine residues by LYN; this creates binding sites for PTPN6/SHP-1.

    Similarity:
    Contains 3 SRCR domains.

    SWISS:
    P06127

    Gene ID:
    921

    Database links:

    Entrez Gene: 921 Human

    Omim: 153340 Human

    SwissProt: P06127 Human

    Unigene: 58685 Human



    產(chǎn)品圖片
    Sample: Lane 1: Human MOLT4 cell lysates Lane 2: Human Raji cell lysates Primary: Anti-CD5 (bsm-33077M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 55 kDa Observed band size: 60 kDa
    Paraformaldehyde-fixed, paraffin embedded (human spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CD5) Monoclonal Antibody, Unconjugated (bsm-33077M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
    Paraformaldehyde-fixed, paraffin embedded (human lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CD5) Monoclonal Antibody, Unconjugated (bsm-33077M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
    Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CD5) Monoclonal Antibody, Unconjugated (bsm-33077M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
    Negative sample:Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle);Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CD5) Monoclonal Antibody, Unconjugated (bsm-33077M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
    Blank control(black line):Jurkat. Primary Antibody (green line): Mouse Anti-CD5 antibody (bsm-33077M) Dilution:1ug/Test; Secondary Antibody(white blue line): Goat anti-mouse IgG-AF488 Dilution: 0.5ug/Test. Isotype control(orange line): Normal Mouse IgG Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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