| 產(chǎn)品編號 | bs-6218R |
| 英文名稱(chēng) | RAR gamma Rabbit pAb |
| 中文名稱(chēng) | 維甲酸受體γ抗體 |
| 別 名 | NR1B3; Nuclear receptor subfamily 1 group B member 3; RARC; RARG; Retinoic acid receptor gamma 1; Retinoic acid receptor gamma 2; Retinoic acid receptor gamma; Retinoic acid receptor gamma polypeptide; Retinoic acid receptor, gamma; RARG_HUMAN. |
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Specific References (1) | bs-6218R has been referenced in 1 publications.
[IF=1.59] Tsai et al. Expression of retinoic acid-binding proteins and retinoic acid receptors in sebaceous cell carcinoma of the eyelids. (2015) BMC.Ophthalmol. 15:142 IHC ; Human.
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| 研究領(lǐng)域 | 腫瘤 細胞生物 染色質(zhì)和核信號 信號轉導 轉錄調節因子 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human (predicted: Mouse,Rat,Rabbit,Pig,Cow,Dog,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000,ICC/IF=1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 50 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Retinoic Acid Receptor gamma: 151-250/454 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Retinoic acid, a metabolite of vitamin A, is necessary for normal organogenesis but acts as a teratogen at high levels during embryonic and fetal development. Retinoic acid functions through its interaction with the nuclear protein, retinoic acid receptor (RAR). RAR belongs to the steroid and thyroid hormone superfamily of nuclear receptor proteins which exert their effects by binding to specific DNA response elements, thus regulating gene expression in target cells. RAR exists as three major subtypes: alpha, beta and gamma. Subunit: Homodimer. Heterodimer with a RAR molecule (By similarity). Binds DNA preferentially as a RAR/RXR heterodimer. Subcellular Location: Nucleus. Similarity: Belongs to the nuclear hormone receptor family. NR2 subfamily. Contains 1 nuclear receptor DNA-binding domain. SWISS: P13631 Gene ID: 5916 Database links: Entrez Gene: 5916 Human Entrez Gene: 19411 Mouse Omim: 180190 Human SwissProt: P13631 Human SwissProt: P18911 Mouse Unigene: 1497 Human Unigene: 733399 Human Unigene: 1273 Mouse Unigene: 198797 Rat |
| 產(chǎn)品圖片 |
Sample:
293T(Human) Cell Lysate at 30 ug
A431(Human) Cell Lysate at 30 ug
Raji(Human) Cell Lysate at 30 ug
NIH/3T3(Mouse) Cell Lysate at 30 ug
Primary: Anti-RAR gamma (bs-6218R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 55 kD
Sample:
Lane 1: Human MCF-7 cell lysates
Lane 2: Human HeLa cell lysates
Lane 3: Human A549 cell lysates
Primary: Anti-RAR gamma (bs-6218R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 48 kDa
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (RAR gamma) polyclonal Antibody, Unconjugated (bs-6218R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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